IFOM

Makoto Hayashi Lab

Makoto Hayashi

Makoto Hayashi

Makoto earned his Bachelor of Science, Master of Science and his Ph.D. from Osaka University under the supervision of Dr. Hisao Masukata. During this period, he studied the mechanism of DNA replication in fission yeast and unexpectedly found that fission yeast heterochromatin protein Swi6/HP1 positively regulates DNA replication firing at pericentromere region. read more

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Chromosome Instabilities

The ends of chromosomes are protected from unwanted enzymatic reactions by telomeres, structures composed of simple repeat sequences and associated proteins. In normal human somatic cells, telomere shortening during DNA replication eventually causes deprotection of telomeres, which activates DNA Damage Response (DDR) and halts cell division (replicative senescence). read more

Laboratory location: Kyoto, Japan

Recent publications

  1. V. Pragathi Masamsetti, Ronnie Ren Jie Low, Ka Sin Mak, Aisling O’Connor, Chris D. Riffkin, Noa Lamm, Laure Crabbe, Jan Karlseder, David C.S. Huang, Makoto T. Hayashi and Anthony J. Cesare
    Replication stress induces mitotic death through parallel pathways regulated by WAPL and telomere deprotection
    Nature Communications, 2019, September 17; 10(1): 4224

  2. Makoto T. Hayashi, Anthony J. Cesare, Teresa Rivera and Jan Karlseder
    Cell Death During Crisis Is Mediated by Mitotic Telomere Deprotection
    Nature, 2015, June 25; 522: 492-496 (selected for the journal cover)

  3. Anthony J. Cesare, Makoto T. Hayashi, Laure Crabbe, and Jan Karlseder
    The Telomere Deprotection Response Is Functionally Distinct from the Genomic DNA Damage Response
    Molecular Cell, 2013, July 25; 51(2): 141-155

  4. Makoto T. Hayashi, Anthony J. Cesare, James A. J. Fitzpatrick, Eros Lazzerini-Denchi and Jan Karlseder
    A Telomere Dependent DNA Damage Checkpoint Induced by Prolonged Mitotic Arrest
    Nature Structural & Molecular Biology, 2012 Mar 11; 19(4): 387-394 (selected for the journal cover)

Collaborations

Dr. Anthony Cesare (Children’s Medical Research Institute)
Dr. John Maciejowski (Sloan Kettering Institute)
Dr. Tomohiko Taguchi (Tohoku University)
Dr. Yasukazu Daigaku (Tohoku University)
Dr. Toyoaki Natsume (Kanemaki lab, National Institute of Genetics)

Group Members

Research Assistants
Mari Tsuboi
Postdocs
Shoma Ishikawa
PhD Students
Diana Romero Zamora
Master Students
Naoya Koshizuka